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resource source identifier antibodies rabbit anti tbx5 sino biological  (Sino Biological)


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    Sino Biological resource source identifier antibodies rabbit anti tbx5 sino biological
    Resource Source Identifier Antibodies Rabbit Anti Tbx5 Sino Biological, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antigen+affinity/TBX5+Antibody%2C+Rabbit+PAb%2C+Antigen+Affinity+Purified/pm42776841-182-2-8
    Average 93 stars, based on 2 article reviews
    resource source identifier antibodies rabbit anti tbx5 sino biological - by Bioz Stars, 2026-10
    93/100 stars

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    Affinity Purification:

    Article Title: Compositions and vaccines for treating and/or preventing coronavirus variant infections and methods of using the same
    Article Snippet: SARS-CoV-2 (COVID-19) spike antibody against the S1 and S2 subunits, were purchased from Genetex (Cat. #GTX135356 and #GTX632604) for western blot confirmation of S protein within EDVTM. .. SARS-CoV-2 (2019-nCoV) spike RBD rabbit PAb, antigen affinity purified (Cat. #40592-T62, Sino Biological) was used for quantitation of the S protein within EDVs using ELISA. .. Cell lines: JAWSII mouse bone marrow derived dendritic cells (ATCC© CRL-11904TM) were grown in α-minimum essential medium with ribonucleosides and deoxyribonucleosides (4 mM L-glut, 1 mM Sodium Pyruvate, 5 ng/ml GMCSF and 20% FBS) at 37° C., 5% CO2.

    Article Title: Single amino acid substitution at position 614 in SARS-CoV-2 Spike Protein alters viral assembly and infectivity
    Article Snippet: .. GoldBand Plus 3-color Regular Range Protein Marker(8–180 kDa) (YEASEN, #20350ES72); SARS-CoV-2 (2019-nCoV) Spike RBD Antibody, Rabbit PAb, Antigen Affinity Purified (Sinobiological, # 40,592-T62); SARS-CoV-2 (2019-nCoV) Spike S2 Antibody, Rabbit PAb, Antigen Affinity Purified (Sinobiological, #40,590-T62); GFP (4B10) Mouse mAb (CST, #2955); Flag (DYKDDDDK) tag Polyclonal Antibody (Proteintech, #20,543–1-AP); Human Immunodeficiency Virus type 1 (HIV-1) Gag-p24 Antibody, Rabbit PAb, Antigen Affinity Purified (Sinobiological, #11,695-RP02); Myc-Tag (9B11) Mouse mAb (CST, #2276); GAPDH Mouse Monoclonal Antibody (Origene, #OTI2D9); β-actin Polyclonal Antibody (Proteintech, #20,536–1-AP). .. The HEK293T and HeLa cell lines were cultured in Dulbecco's modified Eagle medium (DMEM; HyClone, USA) supplemented with 10 % FBS and 1 % penicillin-streptomycin and kindly provided by Stem Cell Bank, Chinese Academy of Sciences.

    Article Title: Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that clamp the spike at its base
    Article Snippet: .. Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight - Alexa Fluor® 700 anti-mouse CD45 Antibody; BioLegend; catalog number 103127; clone 30-F11 - MHC Class II (I-A/I-E) Monoclonal Antibody, APC-eFluorTM 780, eBioscienceTM; Thermo Fisher Scientific; catalog number 47-5321-82; - clone M5/114.15.2 - CD3e Monoclonal Antibody (145-2C11); PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0031-81; clone 145-2c11 - PE/Cyanine5 anti-mouse NK-1.1 antibody; Biolegend; catalog number 108713; clone PK136 - CD19 Monoclonel Antibody (eBIO1D3 (1D3)), PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0193-82; clone 1D3 - CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscienceTM; Thermo Fisher Scientific; catalog number 25-0114-82; clone N418 - SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse Mab; Sino Biological; catalog number 40143-MM05; Monoclonal Mouse IgG1 Clone #05 - SARS-CoV-2 (2019-nCoV) Spike Antibody, Rabbit PAb, Antigen Affinity Purified; Sino Biological; catalog number 40591-T62 The strep-tag antibody (Qiagen; catalog number 34850) was previously not validated for flow cytometry, but was used with appropriate controls (Fig. 8F) FreeStyleTM 293-F cells (purchased from Thermo Fisher Scientific) HEK293-S cells (purchased from Thermo Fisher Scientific) HEK293T cells (purchased from ATCC) Vero E6 cells (purchased from ATCC) ExpiCHO-S cells (purchased from GIBCO) Vero E6-TMPRSS2 cells that stably express human TMPRSS2 (National Institute for Biological Standards and Control (NIBSC) Research Reagent Repository, JCRB1819) None of the used cell lines were authenticated after acquiring them. ..

    Quantitation Assay:

    Article Title: Compositions and vaccines for treating and/or preventing coronavirus variant infections and methods of using the same
    Article Snippet: SARS-CoV-2 (COVID-19) spike antibody against the S1 and S2 subunits, were purchased from Genetex (Cat. #GTX135356 and #GTX632604) for western blot confirmation of S protein within EDVTM. .. SARS-CoV-2 (2019-nCoV) spike RBD rabbit PAb, antigen affinity purified (Cat. #40592-T62, Sino Biological) was used for quantitation of the S protein within EDVs using ELISA. .. Cell lines: JAWSII mouse bone marrow derived dendritic cells (ATCC© CRL-11904TM) were grown in α-minimum essential medium with ribonucleosides and deoxyribonucleosides (4 mM L-glut, 1 mM Sodium Pyruvate, 5 ng/ml GMCSF and 20% FBS) at 37° C., 5% CO2.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Compositions and vaccines for treating and/or preventing coronavirus variant infections and methods of using the same
    Article Snippet: SARS-CoV-2 (COVID-19) spike antibody against the S1 and S2 subunits, were purchased from Genetex (Cat. #GTX135356 and #GTX632604) for western blot confirmation of S protein within EDVTM. .. SARS-CoV-2 (2019-nCoV) spike RBD rabbit PAb, antigen affinity purified (Cat. #40592-T62, Sino Biological) was used for quantitation of the S protein within EDVs using ELISA. .. Cell lines: JAWSII mouse bone marrow derived dendritic cells (ATCC© CRL-11904TM) were grown in α-minimum essential medium with ribonucleosides and deoxyribonucleosides (4 mM L-glut, 1 mM Sodium Pyruvate, 5 ng/ml GMCSF and 20% FBS) at 37° C., 5% CO2.

    Marker:

    Article Title: Single amino acid substitution at position 614 in SARS-CoV-2 Spike Protein alters viral assembly and infectivity
    Article Snippet: .. GoldBand Plus 3-color Regular Range Protein Marker(8–180 kDa) (YEASEN, #20350ES72); SARS-CoV-2 (2019-nCoV) Spike RBD Antibody, Rabbit PAb, Antigen Affinity Purified (Sinobiological, # 40,592-T62); SARS-CoV-2 (2019-nCoV) Spike S2 Antibody, Rabbit PAb, Antigen Affinity Purified (Sinobiological, #40,590-T62); GFP (4B10) Mouse mAb (CST, #2955); Flag (DYKDDDDK) tag Polyclonal Antibody (Proteintech, #20,543–1-AP); Human Immunodeficiency Virus type 1 (HIV-1) Gag-p24 Antibody, Rabbit PAb, Antigen Affinity Purified (Sinobiological, #11,695-RP02); Myc-Tag (9B11) Mouse mAb (CST, #2276); GAPDH Mouse Monoclonal Antibody (Origene, #OTI2D9); β-actin Polyclonal Antibody (Proteintech, #20,536–1-AP). .. The HEK293T and HeLa cell lines were cultured in Dulbecco's modified Eagle medium (DMEM; HyClone, USA) supplemented with 10 % FBS and 1 % penicillin-streptomycin and kindly provided by Stem Cell Bank, Chinese Academy of Sciences.

    Virus:

    Article Title: Single amino acid substitution at position 614 in SARS-CoV-2 Spike Protein alters viral assembly and infectivity
    Article Snippet: .. GoldBand Plus 3-color Regular Range Protein Marker(8–180 kDa) (YEASEN, #20350ES72); SARS-CoV-2 (2019-nCoV) Spike RBD Antibody, Rabbit PAb, Antigen Affinity Purified (Sinobiological, # 40,592-T62); SARS-CoV-2 (2019-nCoV) Spike S2 Antibody, Rabbit PAb, Antigen Affinity Purified (Sinobiological, #40,590-T62); GFP (4B10) Mouse mAb (CST, #2955); Flag (DYKDDDDK) tag Polyclonal Antibody (Proteintech, #20,543–1-AP); Human Immunodeficiency Virus type 1 (HIV-1) Gag-p24 Antibody, Rabbit PAb, Antigen Affinity Purified (Sinobiological, #11,695-RP02); Myc-Tag (9B11) Mouse mAb (CST, #2276); GAPDH Mouse Monoclonal Antibody (Origene, #OTI2D9); β-actin Polyclonal Antibody (Proteintech, #20,536–1-AP). .. The HEK293T and HeLa cell lines were cultured in Dulbecco's modified Eagle medium (DMEM; HyClone, USA) supplemented with 10 % FBS and 1 % penicillin-streptomycin and kindly provided by Stem Cell Bank, Chinese Academy of Sciences.

    other:

    Article Title: Inactivation of SARS-CoV-2 at acidic pH is driven by partial unfolding of spike
    Article Snippet: Human coronavirus (HCoV-229E) Nucleocapsid Antibody, Rabbit PAb, Antigen Affinity Purified, 40640-T62 Sino Biological, RRID:AB_3676912 , , , 1:10,000.

    Article Title: Inactivation of SARS-CoV-2 at acidic pH is driven by partial unfolding of spike
    Article Snippet: Human coronavirus (HCoV-229E) Spike S1 Antibody, Rabbit PAb, Antigen Affinity Purified, 40601-T62 Sino Biological , 1:1000 , 1:100 , .

    Article Title: Inactivation of SARS-CoV-2 at acidic pH is driven by partial unfolding of spike.
    Article Snippet: Digestion was terminated by adding 0.5 μl SUPERase-In RNase Inhibitor (AM2694, Thermo Fisher Scientific) to each sample and incubation for 20min at RT.

    Immunopeptidomics:

    Article Title: Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that clamp the spike at its base
    Article Snippet: .. Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight - Alexa Fluor® 700 anti-mouse CD45 Antibody; BioLegend; catalog number 103127; clone 30-F11 - MHC Class II (I-A/I-E) Monoclonal Antibody, APC-eFluorTM 780, eBioscienceTM; Thermo Fisher Scientific; catalog number 47-5321-82; - clone M5/114.15.2 - CD3e Monoclonal Antibody (145-2C11); PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0031-81; clone 145-2c11 - PE/Cyanine5 anti-mouse NK-1.1 antibody; Biolegend; catalog number 108713; clone PK136 - CD19 Monoclonel Antibody (eBIO1D3 (1D3)), PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0193-82; clone 1D3 - CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscienceTM; Thermo Fisher Scientific; catalog number 25-0114-82; clone N418 - SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse Mab; Sino Biological; catalog number 40143-MM05; Monoclonal Mouse IgG1 Clone #05 - SARS-CoV-2 (2019-nCoV) Spike Antibody, Rabbit PAb, Antigen Affinity Purified; Sino Biological; catalog number 40591-T62 The strep-tag antibody (Qiagen; catalog number 34850) was previously not validated for flow cytometry, but was used with appropriate controls (Fig. 8F) FreeStyleTM 293-F cells (purchased from Thermo Fisher Scientific) HEK293-S cells (purchased from Thermo Fisher Scientific) HEK293T cells (purchased from ATCC) Vero E6 cells (purchased from ATCC) ExpiCHO-S cells (purchased from GIBCO) Vero E6-TMPRSS2 cells that stably express human TMPRSS2 (National Institute for Biological Standards and Control (NIBSC) Research Reagent Repository, JCRB1819) None of the used cell lines were authenticated after acquiring them. ..

    Strep-tag:

    Article Title: Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that clamp the spike at its base
    Article Snippet: .. Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight - Alexa Fluor® 700 anti-mouse CD45 Antibody; BioLegend; catalog number 103127; clone 30-F11 - MHC Class II (I-A/I-E) Monoclonal Antibody, APC-eFluorTM 780, eBioscienceTM; Thermo Fisher Scientific; catalog number 47-5321-82; - clone M5/114.15.2 - CD3e Monoclonal Antibody (145-2C11); PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0031-81; clone 145-2c11 - PE/Cyanine5 anti-mouse NK-1.1 antibody; Biolegend; catalog number 108713; clone PK136 - CD19 Monoclonel Antibody (eBIO1D3 (1D3)), PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0193-82; clone 1D3 - CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscienceTM; Thermo Fisher Scientific; catalog number 25-0114-82; clone N418 - SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse Mab; Sino Biological; catalog number 40143-MM05; Monoclonal Mouse IgG1 Clone #05 - SARS-CoV-2 (2019-nCoV) Spike Antibody, Rabbit PAb, Antigen Affinity Purified; Sino Biological; catalog number 40591-T62 The strep-tag antibody (Qiagen; catalog number 34850) was previously not validated for flow cytometry, but was used with appropriate controls (Fig. 8F) FreeStyleTM 293-F cells (purchased from Thermo Fisher Scientific) HEK293-S cells (purchased from Thermo Fisher Scientific) HEK293T cells (purchased from ATCC) Vero E6 cells (purchased from ATCC) ExpiCHO-S cells (purchased from GIBCO) Vero E6-TMPRSS2 cells that stably express human TMPRSS2 (National Institute for Biological Standards and Control (NIBSC) Research Reagent Repository, JCRB1819) None of the used cell lines were authenticated after acquiring them. ..

    Flow Cytometry:

    Article Title: Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that clamp the spike at its base
    Article Snippet: .. Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight - Alexa Fluor® 700 anti-mouse CD45 Antibody; BioLegend; catalog number 103127; clone 30-F11 - MHC Class II (I-A/I-E) Monoclonal Antibody, APC-eFluorTM 780, eBioscienceTM; Thermo Fisher Scientific; catalog number 47-5321-82; - clone M5/114.15.2 - CD3e Monoclonal Antibody (145-2C11); PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0031-81; clone 145-2c11 - PE/Cyanine5 anti-mouse NK-1.1 antibody; Biolegend; catalog number 108713; clone PK136 - CD19 Monoclonel Antibody (eBIO1D3 (1D3)), PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0193-82; clone 1D3 - CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscienceTM; Thermo Fisher Scientific; catalog number 25-0114-82; clone N418 - SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse Mab; Sino Biological; catalog number 40143-MM05; Monoclonal Mouse IgG1 Clone #05 - SARS-CoV-2 (2019-nCoV) Spike Antibody, Rabbit PAb, Antigen Affinity Purified; Sino Biological; catalog number 40591-T62 The strep-tag antibody (Qiagen; catalog number 34850) was previously not validated for flow cytometry, but was used with appropriate controls (Fig. 8F) FreeStyleTM 293-F cells (purchased from Thermo Fisher Scientific) HEK293-S cells (purchased from Thermo Fisher Scientific) HEK293T cells (purchased from ATCC) Vero E6 cells (purchased from ATCC) ExpiCHO-S cells (purchased from GIBCO) Vero E6-TMPRSS2 cells that stably express human TMPRSS2 (National Institute for Biological Standards and Control (NIBSC) Research Reagent Repository, JCRB1819) None of the used cell lines were authenticated after acquiring them. ..

    Stable Transfection:

    Article Title: Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that clamp the spike at its base
    Article Snippet: .. Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight - Alexa Fluor® 700 anti-mouse CD45 Antibody; BioLegend; catalog number 103127; clone 30-F11 - MHC Class II (I-A/I-E) Monoclonal Antibody, APC-eFluorTM 780, eBioscienceTM; Thermo Fisher Scientific; catalog number 47-5321-82; - clone M5/114.15.2 - CD3e Monoclonal Antibody (145-2C11); PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0031-81; clone 145-2c11 - PE/Cyanine5 anti-mouse NK-1.1 antibody; Biolegend; catalog number 108713; clone PK136 - CD19 Monoclonel Antibody (eBIO1D3 (1D3)), PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0193-82; clone 1D3 - CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscienceTM; Thermo Fisher Scientific; catalog number 25-0114-82; clone N418 - SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse Mab; Sino Biological; catalog number 40143-MM05; Monoclonal Mouse IgG1 Clone #05 - SARS-CoV-2 (2019-nCoV) Spike Antibody, Rabbit PAb, Antigen Affinity Purified; Sino Biological; catalog number 40591-T62 The strep-tag antibody (Qiagen; catalog number 34850) was previously not validated for flow cytometry, but was used with appropriate controls (Fig. 8F) FreeStyleTM 293-F cells (purchased from Thermo Fisher Scientific) HEK293-S cells (purchased from Thermo Fisher Scientific) HEK293T cells (purchased from ATCC) Vero E6 cells (purchased from ATCC) ExpiCHO-S cells (purchased from GIBCO) Vero E6-TMPRSS2 cells that stably express human TMPRSS2 (National Institute for Biological Standards and Control (NIBSC) Research Reagent Repository, JCRB1819) None of the used cell lines were authenticated after acquiring them. ..

    Control:

    Article Title: Ultrapotent SARS coronavirus-neutralizing single-domain antibodies that clamp the spike at its base
    Article Snippet: .. Animals and other research organisms Policy information about studies involving animals; ARRIVE guidelines recommended for reporting animal research, and Sex and Gender in Research Laboratory animals Wild animals Reporting on sex Field-collected samples Ethics oversight - Alexa Fluor® 700 anti-mouse CD45 Antibody; BioLegend; catalog number 103127; clone 30-F11 - MHC Class II (I-A/I-E) Monoclonal Antibody, APC-eFluorTM 780, eBioscienceTM; Thermo Fisher Scientific; catalog number 47-5321-82; - clone M5/114.15.2 - CD3e Monoclonal Antibody (145-2C11); PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0031-81; clone 145-2c11 - PE/Cyanine5 anti-mouse NK-1.1 antibody; Biolegend; catalog number 108713; clone PK136 - CD19 Monoclonel Antibody (eBIO1D3 (1D3)), PE-Cyanine5, eBioscienceTM; Thermo Fisher Scientific; catalog number 15-0193-82; clone 1D3 - CD11c Monoclonal Antibody (N418), PE-Cyanine7, eBioscienceTM; Thermo Fisher Scientific; catalog number 25-0114-82; clone N418 - SARS-CoV/SARS-CoV-2 Nucleocapsid Antibody, Mouse Mab; Sino Biological; catalog number 40143-MM05; Monoclonal Mouse IgG1 Clone #05 - SARS-CoV-2 (2019-nCoV) Spike Antibody, Rabbit PAb, Antigen Affinity Purified; Sino Biological; catalog number 40591-T62 The strep-tag antibody (Qiagen; catalog number 34850) was previously not validated for flow cytometry, but was used with appropriate controls (Fig. 8F) FreeStyleTM 293-F cells (purchased from Thermo Fisher Scientific) HEK293-S cells (purchased from Thermo Fisher Scientific) HEK293T cells (purchased from ATCC) Vero E6 cells (purchased from ATCC) ExpiCHO-S cells (purchased from GIBCO) Vero E6-TMPRSS2 cells that stably express human TMPRSS2 (National Institute for Biological Standards and Control (NIBSC) Research Reagent Repository, JCRB1819) None of the used cell lines were authenticated after acquiring them. ..



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    94
    sino biological 205166-t08
    IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of <t>HCoV-OC43</t> N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
    205166 T08, supplied by sino biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    205166 t08  (Sino Biological)
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    Sino Biological 205166 t08
    IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of <t>HCoV-OC43</t> N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.
    205166 T08, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antigen+affinity/MAGEB10+Antibody%2C+Rabbit+PAb%2C+Antigen+Affinity+Purified/pmc13130648-18-7-4
    Average 94 stars, based on 1 article reviews
    205166 t08 - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

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    IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of HCoV-OC43 N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

    Journal: bioRxiv

    Article Title: Proximity proteomics reveals a role for IFI16 during human coronavirus infection

    doi: 10.64898/2026.04.22.720112

    Figure Lengend Snippet: IFI16 promotes coronavirus infection. ( a) CRISPR-Cas9 technology was used to knock out IFI16 in A549 cells and single cell clones were screened for loss of IFI16 expression using qRT-PCR. Data is representative of two independent experiments. ( b) Immunoblot showing the loss of IFI16 expression in IFI16-KO cell line. ( c) Immunofluorescence assay showing reduced number of HCoV-OC43 N-positive cells upon loss of IFI16. Cells were infected at an MOI of 0.1 and incubated for the indicated length of time before fixation and staining for HCoV-OC43 N protein. Scale bar is 10 um. ( d ) Representative flow cytometry analysis of wildtype (WT) A549 cells and IFI16-KO cells infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 and 0.1 for 12, 24 and 48 h. Data is representative of 3 independent experiments. (e) and (f) Quantification of flow cytometry data in . ( g) Representative flow cytometry analysis of CRISPRi cell lines infected (in quadruplicate) with HCoV-OC43 at an MOI of 0.01 for 12, 24 and 48 h. data in figure. (h) Quantification of flow cytometry data in . Data is representative of two independent experiments. Statistical significance was assessed by ANOVA. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

    Article Snippet: Cells were washed twice with a permeabilization/wash buffer (0.1% saponin and 0.5% BSA in 1X PBS) and stained with a 1:10000 dilution of anti-HCoV-OC43-N rabbit polyclonal antibody (catalogue no. 40643-T62; Sino Biological) for 30 min at 4°C.

    Techniques: Infection, CRISPR, Knock-Out, Single Cell, Clone Assay, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Incubation, Staining, Flow Cytometry