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sars cov 2 nucleocapsid  (Sino Biological)


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    Sino Biological sars cov 2 nucleocapsid
    Infection of iPSC-CMs by <t>SARS-CoV-2</t> in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in Suppl. Fig. 3A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Sars Cov 2 Nucleocapsid, supplied by Sino Biological, used in various techniques. Bioz Stars score: 96/100, based on 157 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 nucleocapsid/product/Sino Biological
    Average 96 stars, based on 157 article reviews
    sars cov 2 nucleocapsid - by Bioz Stars, 2026-02
    96/100 stars

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    1) Product Images from "SARS-CoV-2-infected cardiomyocytes exhibit upregulated necroptosis, but no evidence of mitochondrial permeability transition"

    Article Title: SARS-CoV-2-infected cardiomyocytes exhibit upregulated necroptosis, but no evidence of mitochondrial permeability transition

    Journal: Journal of Molecular and Cellular Cardiology Plus

    doi: 10.1016/j.jmccpl.2025.100833

    Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in Suppl. Fig. 3A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Figure Legend Snippet: Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in Suppl. Fig. 3A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Techniques Used: Infection, In Vitro, Immunofluorescence, Staining, Derivative Assay, Expressing, Western Blot, Activity Assay, Gene Expression

    PDH phosphorylation is unaffected following SARS-CoV2 infection. (A) Cytotoxicity after Cyclosporine A (CsA) treatment [0.5 μM, 2 h] as determined by activity of LDH released into the medium. (B) Quantification of pyruvate dehydrogenase A1 (PDHA1) protein expression and its phosphorylation on serine 293. Values normalized to α-tubulin. (C) Representative Western Blot. (D) Quantification of CI = Complex I, CII = Complex II, CIII = Complex III, CIV = Complex IV and CV = Complex V normalized to α-tubulin. Full western blots in B. (E) Protein expression of the respiratory chain following SARS-CoV-2 infection. Full western blots in B. (F) Apoptosis rate determined by TUNEL staining 24 h post-infection with an MOI of 0.1. (G) Quantification of procaspase 3 and cleaved caspase 3 protein expression. Values normalized to α-tubulin. Corresponding western blots in C. n = 3 iPSC-CM differentiation rounds for all but (A, B), where n = 1 iPSC-CM differentiation round. Statistical significance determined by two-way ANOVA (A, B), Mann-Whitney test (F) or t-test (D, G). p ≤ 0.05 = *, p < 0.0001 = ****. Data are displayed as mean ± SD. PDH phosphorylation is unaffected following SARS-CoV2 infection. (A) Cytotoxicity after Cyclosporine A (CsA) treatment [0.5 μM, 2 h] as determined by activity of LDH released into the medium. (B) Quantification of pyruvate dehydrogenase A1 (PDHA1) protein expression and its phosphorylation on serine 293. Values normalized to α-tubulin. (C) Representative Western Blot. (D) Quantification of CI = Complex I, CII = Complex II, CIII = Complex III, CIV = Complex IV and CV = Complex V normalized to α-tubulin. Full western blots in Suppl. Fig. 3B. (E) Protein expression of the respiratory chain following SARS-CoV-2 infection. Full western blots in Suppl. Fig. 3B. (F) Apoptosis rate determined by TUNEL staining 24 h post-infection with an MOI of 0.1. (G) Quantification of procaspase 3 and cleaved caspase 3 protein expression. Values normalized to α-tubulin. Corresponding western blots in Suppl. Fig. 3C. n = 3 iPSC-CM differentiation rounds for all but (A, B), where n = 1 iPSC-CM differentiation round. Statistical significance determined by two-way ANOVA (A, B), Mann-Whitney test (F) or t-test (D, G). p ≤ 0.05 = *, p < 0.0001 = ****. Data are displayed as mean ± SD.
    Figure Legend Snippet: PDH phosphorylation is unaffected following SARS-CoV2 infection. (A) Cytotoxicity after Cyclosporine A (CsA) treatment [0.5 μM, 2 h] as determined by activity of LDH released into the medium. (B) Quantification of pyruvate dehydrogenase A1 (PDHA1) protein expression and its phosphorylation on serine 293. Values normalized to α-tubulin. (C) Representative Western Blot. (D) Quantification of CI = Complex I, CII = Complex II, CIII = Complex III, CIV = Complex IV and CV = Complex V normalized to α-tubulin. Full western blots in B. (E) Protein expression of the respiratory chain following SARS-CoV-2 infection. Full western blots in B. (F) Apoptosis rate determined by TUNEL staining 24 h post-infection with an MOI of 0.1. (G) Quantification of procaspase 3 and cleaved caspase 3 protein expression. Values normalized to α-tubulin. Corresponding western blots in C. n = 3 iPSC-CM differentiation rounds for all but (A, B), where n = 1 iPSC-CM differentiation round. Statistical significance determined by two-way ANOVA (A, B), Mann-Whitney test (F) or t-test (D, G). p ≤ 0.05 = *, p < 0.0001 = ****. Data are displayed as mean ± SD. PDH phosphorylation is unaffected following SARS-CoV2 infection. (A) Cytotoxicity after Cyclosporine A (CsA) treatment [0.5 μM, 2 h] as determined by activity of LDH released into the medium. (B) Quantification of pyruvate dehydrogenase A1 (PDHA1) protein expression and its phosphorylation on serine 293. Values normalized to α-tubulin. (C) Representative Western Blot. (D) Quantification of CI = Complex I, CII = Complex II, CIII = Complex III, CIV = Complex IV and CV = Complex V normalized to α-tubulin. Full western blots in Suppl. Fig. 3B. (E) Protein expression of the respiratory chain following SARS-CoV-2 infection. Full western blots in Suppl. Fig. 3B. (F) Apoptosis rate determined by TUNEL staining 24 h post-infection with an MOI of 0.1. (G) Quantification of procaspase 3 and cleaved caspase 3 protein expression. Values normalized to α-tubulin. Corresponding western blots in Suppl. Fig. 3C. n = 3 iPSC-CM differentiation rounds for all but (A, B), where n = 1 iPSC-CM differentiation round. Statistical significance determined by two-way ANOVA (A, B), Mann-Whitney test (F) or t-test (D, G). p ≤ 0.05 = *, p < 0.0001 = ****. Data are displayed as mean ± SD.

    Techniques Used: Phospho-proteomics, Infection, Activity Assay, Expressing, Western Blot, TUNEL Assay, Staining, MANN-WHITNEY

    Increased necroptosis following SARS-CoV-2 infection. (A) Cytotoxicity after Necrostatin-1 (Nec-1) [2.5 μM] or MCC950 [0.05 μM] treatment as determined by activity of LDH released into the medium. (B) Spike- (C) RIP3 and (D) NLR family pyrin domain containing 3 (NLRP3) inflammasome gene expression following SARS-CoV-2 infection. Gene expression normalized to GAPDH. (E) Quantification of RIP3 and RIP3-S227 protein expression after Nec-1 treatment. (F) Representative WB for RIP3 and RIP3-S227 following SARS-CoV-2 infection. Full western blots in D. (G) Quantification of MLKL and MLKL-S358 protein expression with a representative WB (H). Full western blots in E. n = 3 iPSC-CM differentiation rounds, statistical significance determined by two-way ANOVA (A) or Kruskal-Wallis test (B–E) or Mann-Whitney (G). p < 0.01 = **, p < 0.0001 = ****. Data are displayed as mean ± SD. Increased necroptosis following SARS-CoV-2 infection. (A) Cytotoxicity after Necrostatin-1 (Nec-1) [2.5 μM] or MCC950 [0.05 μM] treatment as determined by activity of LDH released into the medium. (B) Spike- (C) RIP3 and (D) NLR family pyrin domain containing 3 (NLRP3) inflammasome gene expression following SARS-CoV-2 infection. Gene expression normalized to GAPDH. (E) Quantification of RIP3 and RIP3-S227 protein expression after Nec-1 treatment. (F) Representative WB for RIP3 and RIP3-S227 following SARS-CoV-2 infection. Full western blots in Suppl. Fig. 3D. (G) Quantification of MLKL and MLKL-S358 protein expression with a representative WB (H). Full western blots in Suppl. Fig. 3E. n = 3 iPSC-CM differentiation rounds, statistical significance determined by two-way ANOVA (A) or Kruskal-Wallis test (B–E) or Mann-Whitney (G). p < 0.01 = **, p < 0.0001 = ****. Data are displayed as mean ± SD.
    Figure Legend Snippet: Increased necroptosis following SARS-CoV-2 infection. (A) Cytotoxicity after Necrostatin-1 (Nec-1) [2.5 μM] or MCC950 [0.05 μM] treatment as determined by activity of LDH released into the medium. (B) Spike- (C) RIP3 and (D) NLR family pyrin domain containing 3 (NLRP3) inflammasome gene expression following SARS-CoV-2 infection. Gene expression normalized to GAPDH. (E) Quantification of RIP3 and RIP3-S227 protein expression after Nec-1 treatment. (F) Representative WB for RIP3 and RIP3-S227 following SARS-CoV-2 infection. Full western blots in D. (G) Quantification of MLKL and MLKL-S358 protein expression with a representative WB (H). Full western blots in E. n = 3 iPSC-CM differentiation rounds, statistical significance determined by two-way ANOVA (A) or Kruskal-Wallis test (B–E) or Mann-Whitney (G). p < 0.01 = **, p < 0.0001 = ****. Data are displayed as mean ± SD. Increased necroptosis following SARS-CoV-2 infection. (A) Cytotoxicity after Necrostatin-1 (Nec-1) [2.5 μM] or MCC950 [0.05 μM] treatment as determined by activity of LDH released into the medium. (B) Spike- (C) RIP3 and (D) NLR family pyrin domain containing 3 (NLRP3) inflammasome gene expression following SARS-CoV-2 infection. Gene expression normalized to GAPDH. (E) Quantification of RIP3 and RIP3-S227 protein expression after Nec-1 treatment. (F) Representative WB for RIP3 and RIP3-S227 following SARS-CoV-2 infection. Full western blots in Suppl. Fig. 3D. (G) Quantification of MLKL and MLKL-S358 protein expression with a representative WB (H). Full western blots in Suppl. Fig. 3E. n = 3 iPSC-CM differentiation rounds, statistical significance determined by two-way ANOVA (A) or Kruskal-Wallis test (B–E) or Mann-Whitney (G). p < 0.01 = **, p < 0.0001 = ****. Data are displayed as mean ± SD.

    Techniques Used: Infection, Activity Assay, Gene Expression, Expressing, Western Blot, MANN-WHITNEY



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    Infection of iPSC-CMs by <t>SARS-CoV-2</t> in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in Suppl. Fig. 3A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Anti Mers Cov S2 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti s tag  (Sino Biological)
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    Infection of iPSC-CMs by <t>SARS-CoV-2</t> in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in Suppl. Fig. 3A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    Anti S Tag, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Infection of iPSC-CMs by <t>SARS-CoV-2</t> in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in Suppl. Fig. 3A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    40041 T62, supplied by sino biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Infection of iPSC-CMs by <t>SARS-CoV-2</t> in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in Suppl. Fig. 3A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
    40041 T62, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Infection of iPSC-CMs by <t>SARS-CoV-2</t> in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in Suppl. Fig. 3A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
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    Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in Suppl. Fig. 3A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Journal of Molecular and Cellular Cardiology Plus

    Article Title: SARS-CoV-2-infected cardiomyocytes exhibit upregulated necroptosis, but no evidence of mitochondrial permeability transition

    doi: 10.1016/j.jmccpl.2025.100833

    Figure Lengend Snippet: Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Infection of iPSC-CMs by SARS-CoV-2 in vitro results in increased cellular cytotoxicity. (A) Immunofluorescence staining shows infection of iPSC-derived cardiomyocytes. Nuclei (blue), α-Actinin (grey) and Nucleocapsid (red). Scale bar: 50 μm.(B) Quantification of infected cells at 24 h post-infection. (C) Infection of iPSC-derived cardiomyocytes is confirmed by expression of SARS-CoV-2 Spike RNA. (D) Western blot analysis confirms Spike Protein expression, quantified in (E). Full western blots in Suppl. Fig. 3A. (F) Cytotoxicity upon SARS-CoV-2 infection determined by activity of LDH released into the medium. Protein expression is normalized to β-actin, gene expression is normalized to GAPDH. n = 3 iPSC-CM differentiation rounds for all but (B), where n = 1 iPSC-CM differentiation round, statistical significance determined by Wilcoxon test (B), Kruskal-Wallis test (C, D), students t -test (F). p < 0.0001 = ****. Data are displayed as mean ± SD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: The following antibodies were used in a 1:500 dilution: α-Actinin (Sigma, #A7811) and SARS-CoV-2 Nucleocapsid (SinoBiological, #40143-T62).

    Techniques: Infection, In Vitro, Immunofluorescence, Staining, Derivative Assay, Expressing, Western Blot, Activity Assay, Gene Expression

    PDH phosphorylation is unaffected following SARS-CoV2 infection. (A) Cytotoxicity after Cyclosporine A (CsA) treatment [0.5 μM, 2 h] as determined by activity of LDH released into the medium. (B) Quantification of pyruvate dehydrogenase A1 (PDHA1) protein expression and its phosphorylation on serine 293. Values normalized to α-tubulin. (C) Representative Western Blot. (D) Quantification of CI = Complex I, CII = Complex II, CIII = Complex III, CIV = Complex IV and CV = Complex V normalized to α-tubulin. Full western blots in B. (E) Protein expression of the respiratory chain following SARS-CoV-2 infection. Full western blots in B. (F) Apoptosis rate determined by TUNEL staining 24 h post-infection with an MOI of 0.1. (G) Quantification of procaspase 3 and cleaved caspase 3 protein expression. Values normalized to α-tubulin. Corresponding western blots in C. n = 3 iPSC-CM differentiation rounds for all but (A, B), where n = 1 iPSC-CM differentiation round. Statistical significance determined by two-way ANOVA (A, B), Mann-Whitney test (F) or t-test (D, G). p ≤ 0.05 = *, p < 0.0001 = ****. Data are displayed as mean ± SD. PDH phosphorylation is unaffected following SARS-CoV2 infection. (A) Cytotoxicity after Cyclosporine A (CsA) treatment [0.5 μM, 2 h] as determined by activity of LDH released into the medium. (B) Quantification of pyruvate dehydrogenase A1 (PDHA1) protein expression and its phosphorylation on serine 293. Values normalized to α-tubulin. (C) Representative Western Blot. (D) Quantification of CI = Complex I, CII = Complex II, CIII = Complex III, CIV = Complex IV and CV = Complex V normalized to α-tubulin. Full western blots in Suppl. Fig. 3B. (E) Protein expression of the respiratory chain following SARS-CoV-2 infection. Full western blots in Suppl. Fig. 3B. (F) Apoptosis rate determined by TUNEL staining 24 h post-infection with an MOI of 0.1. (G) Quantification of procaspase 3 and cleaved caspase 3 protein expression. Values normalized to α-tubulin. Corresponding western blots in Suppl. Fig. 3C. n = 3 iPSC-CM differentiation rounds for all but (A, B), where n = 1 iPSC-CM differentiation round. Statistical significance determined by two-way ANOVA (A, B), Mann-Whitney test (F) or t-test (D, G). p ≤ 0.05 = *, p < 0.0001 = ****. Data are displayed as mean ± SD.

    Journal: Journal of Molecular and Cellular Cardiology Plus

    Article Title: SARS-CoV-2-infected cardiomyocytes exhibit upregulated necroptosis, but no evidence of mitochondrial permeability transition

    doi: 10.1016/j.jmccpl.2025.100833

    Figure Lengend Snippet: PDH phosphorylation is unaffected following SARS-CoV2 infection. (A) Cytotoxicity after Cyclosporine A (CsA) treatment [0.5 μM, 2 h] as determined by activity of LDH released into the medium. (B) Quantification of pyruvate dehydrogenase A1 (PDHA1) protein expression and its phosphorylation on serine 293. Values normalized to α-tubulin. (C) Representative Western Blot. (D) Quantification of CI = Complex I, CII = Complex II, CIII = Complex III, CIV = Complex IV and CV = Complex V normalized to α-tubulin. Full western blots in B. (E) Protein expression of the respiratory chain following SARS-CoV-2 infection. Full western blots in B. (F) Apoptosis rate determined by TUNEL staining 24 h post-infection with an MOI of 0.1. (G) Quantification of procaspase 3 and cleaved caspase 3 protein expression. Values normalized to α-tubulin. Corresponding western blots in C. n = 3 iPSC-CM differentiation rounds for all but (A, B), where n = 1 iPSC-CM differentiation round. Statistical significance determined by two-way ANOVA (A, B), Mann-Whitney test (F) or t-test (D, G). p ≤ 0.05 = *, p < 0.0001 = ****. Data are displayed as mean ± SD. PDH phosphorylation is unaffected following SARS-CoV2 infection. (A) Cytotoxicity after Cyclosporine A (CsA) treatment [0.5 μM, 2 h] as determined by activity of LDH released into the medium. (B) Quantification of pyruvate dehydrogenase A1 (PDHA1) protein expression and its phosphorylation on serine 293. Values normalized to α-tubulin. (C) Representative Western Blot. (D) Quantification of CI = Complex I, CII = Complex II, CIII = Complex III, CIV = Complex IV and CV = Complex V normalized to α-tubulin. Full western blots in Suppl. Fig. 3B. (E) Protein expression of the respiratory chain following SARS-CoV-2 infection. Full western blots in Suppl. Fig. 3B. (F) Apoptosis rate determined by TUNEL staining 24 h post-infection with an MOI of 0.1. (G) Quantification of procaspase 3 and cleaved caspase 3 protein expression. Values normalized to α-tubulin. Corresponding western blots in Suppl. Fig. 3C. n = 3 iPSC-CM differentiation rounds for all but (A, B), where n = 1 iPSC-CM differentiation round. Statistical significance determined by two-way ANOVA (A, B), Mann-Whitney test (F) or t-test (D, G). p ≤ 0.05 = *, p < 0.0001 = ****. Data are displayed as mean ± SD.

    Article Snippet: The following antibodies were used in a 1:500 dilution: α-Actinin (Sigma, #A7811) and SARS-CoV-2 Nucleocapsid (SinoBiological, #40143-T62).

    Techniques: Phospho-proteomics, Infection, Activity Assay, Expressing, Western Blot, TUNEL Assay, Staining, MANN-WHITNEY

    Increased necroptosis following SARS-CoV-2 infection. (A) Cytotoxicity after Necrostatin-1 (Nec-1) [2.5 μM] or MCC950 [0.05 μM] treatment as determined by activity of LDH released into the medium. (B) Spike- (C) RIP3 and (D) NLR family pyrin domain containing 3 (NLRP3) inflammasome gene expression following SARS-CoV-2 infection. Gene expression normalized to GAPDH. (E) Quantification of RIP3 and RIP3-S227 protein expression after Nec-1 treatment. (F) Representative WB for RIP3 and RIP3-S227 following SARS-CoV-2 infection. Full western blots in D. (G) Quantification of MLKL and MLKL-S358 protein expression with a representative WB (H). Full western blots in E. n = 3 iPSC-CM differentiation rounds, statistical significance determined by two-way ANOVA (A) or Kruskal-Wallis test (B–E) or Mann-Whitney (G). p < 0.01 = **, p < 0.0001 = ****. Data are displayed as mean ± SD. Increased necroptosis following SARS-CoV-2 infection. (A) Cytotoxicity after Necrostatin-1 (Nec-1) [2.5 μM] or MCC950 [0.05 μM] treatment as determined by activity of LDH released into the medium. (B) Spike- (C) RIP3 and (D) NLR family pyrin domain containing 3 (NLRP3) inflammasome gene expression following SARS-CoV-2 infection. Gene expression normalized to GAPDH. (E) Quantification of RIP3 and RIP3-S227 protein expression after Nec-1 treatment. (F) Representative WB for RIP3 and RIP3-S227 following SARS-CoV-2 infection. Full western blots in Suppl. Fig. 3D. (G) Quantification of MLKL and MLKL-S358 protein expression with a representative WB (H). Full western blots in Suppl. Fig. 3E. n = 3 iPSC-CM differentiation rounds, statistical significance determined by two-way ANOVA (A) or Kruskal-Wallis test (B–E) or Mann-Whitney (G). p < 0.01 = **, p < 0.0001 = ****. Data are displayed as mean ± SD.

    Journal: Journal of Molecular and Cellular Cardiology Plus

    Article Title: SARS-CoV-2-infected cardiomyocytes exhibit upregulated necroptosis, but no evidence of mitochondrial permeability transition

    doi: 10.1016/j.jmccpl.2025.100833

    Figure Lengend Snippet: Increased necroptosis following SARS-CoV-2 infection. (A) Cytotoxicity after Necrostatin-1 (Nec-1) [2.5 μM] or MCC950 [0.05 μM] treatment as determined by activity of LDH released into the medium. (B) Spike- (C) RIP3 and (D) NLR family pyrin domain containing 3 (NLRP3) inflammasome gene expression following SARS-CoV-2 infection. Gene expression normalized to GAPDH. (E) Quantification of RIP3 and RIP3-S227 protein expression after Nec-1 treatment. (F) Representative WB for RIP3 and RIP3-S227 following SARS-CoV-2 infection. Full western blots in D. (G) Quantification of MLKL and MLKL-S358 protein expression with a representative WB (H). Full western blots in E. n = 3 iPSC-CM differentiation rounds, statistical significance determined by two-way ANOVA (A) or Kruskal-Wallis test (B–E) or Mann-Whitney (G). p < 0.01 = **, p < 0.0001 = ****. Data are displayed as mean ± SD. Increased necroptosis following SARS-CoV-2 infection. (A) Cytotoxicity after Necrostatin-1 (Nec-1) [2.5 μM] or MCC950 [0.05 μM] treatment as determined by activity of LDH released into the medium. (B) Spike- (C) RIP3 and (D) NLR family pyrin domain containing 3 (NLRP3) inflammasome gene expression following SARS-CoV-2 infection. Gene expression normalized to GAPDH. (E) Quantification of RIP3 and RIP3-S227 protein expression after Nec-1 treatment. (F) Representative WB for RIP3 and RIP3-S227 following SARS-CoV-2 infection. Full western blots in Suppl. Fig. 3D. (G) Quantification of MLKL and MLKL-S358 protein expression with a representative WB (H). Full western blots in Suppl. Fig. 3E. n = 3 iPSC-CM differentiation rounds, statistical significance determined by two-way ANOVA (A) or Kruskal-Wallis test (B–E) or Mann-Whitney (G). p < 0.01 = **, p < 0.0001 = ****. Data are displayed as mean ± SD.

    Article Snippet: The following antibodies were used in a 1:500 dilution: α-Actinin (Sigma, #A7811) and SARS-CoV-2 Nucleocapsid (SinoBiological, #40143-T62).

    Techniques: Infection, Activity Assay, Gene Expression, Expressing, Western Blot, MANN-WHITNEY